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human lung adenocarcinoma a549  (ATCC)


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    Structured Review

    ATCC human lung adenocarcinoma a549
    Human Lung Adenocarcinoma A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 32274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+adenocarcinoma/A549/pm42883230-217-0-4
    Average 99 stars, based on 32274 article reviews
    human lung adenocarcinoma a549 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC.
    Article Snippet: .. MATERIALS AND METHODS Cell culture, cell stimulation and cell line authentication Cells were incubated at 37 °C and 5% CO2 in a humidified incubator and centrifuged at 300 × g for 5 min. Human adenocarcinoma (A549, H838, H1650, H1975) and squamous cell carcinoma (H520) cell lines were purchased from the American Type Culture Collection (ATCC) and JURKAT cells from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany). .. Cells were maintained in RPMI-1640 supplemented with 10% FCS, 1% penicillin/streptomycin, and 1% stable glutamine (PAN Biotech, Aidenbach, Germany).

    Article Title: Impact of Sample Preparation Methods on Single-Cell X-ray Microscopy and Light Elemental Analysis Evaluated by Combined Low Energy X-ray Fluorescence, STXM and AFM
    Article Snippet: .. HEC-1-A endometrial cells from human adenocarcinoma (ATCC HTB-112) were cultured in McCoy’s 5a Medium supplemented with 10% foetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma Aldrich, Milan, Italy). .. For the experiments 50.000 cells were seeded in 12 multiwell plates containing silicon nitride membranes (100 nm thick; Silson, Southam, UK).

    Article Title: TPGS and Doca dual-modified mesoporous silica nanoparticle-supported lipid bilayers enhance the efficient delivery and in vivo absorption of Coenzyme Q10
    Article Snippet: Coenzyme Q10 (CoQ10) has a wide range of nutraceutical functions and therapeutic values, nevertheless, its biomedical application as a health product or drug is hampered by the low absorption efficiency and bioavailability.. In the present study, the hybrid mesoporous silica nanoparticle-supported lipid bilayers (LMSNs) as nanocarriers for CoQ10 are prepared by fusing the phospholipid membrane onto MSNs cores.. Further, LMSNs are functionalized with D-α-tocopherol polyethylene glycol 1000 succinate (TPGS), deoxycholic acid (Doca) and chitosan (CS) sequentially, to obtain the TPGS-LMSNs and DCs-TPGS-LMSNs samples, respectively.

    Cell Stimulation:

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC.
    Article Snippet: .. MATERIALS AND METHODS Cell culture, cell stimulation and cell line authentication Cells were incubated at 37 °C and 5% CO2 in a humidified incubator and centrifuged at 300 × g for 5 min. Human adenocarcinoma (A549, H838, H1650, H1975) and squamous cell carcinoma (H520) cell lines were purchased from the American Type Culture Collection (ATCC) and JURKAT cells from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany). .. Cells were maintained in RPMI-1640 supplemented with 10% FCS, 1% penicillin/streptomycin, and 1% stable glutamine (PAN Biotech, Aidenbach, Germany).

    Incubation:

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC.
    Article Snippet: .. MATERIALS AND METHODS Cell culture, cell stimulation and cell line authentication Cells were incubated at 37 °C and 5% CO2 in a humidified incubator and centrifuged at 300 × g for 5 min. Human adenocarcinoma (A549, H838, H1650, H1975) and squamous cell carcinoma (H520) cell lines were purchased from the American Type Culture Collection (ATCC) and JURKAT cells from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany). .. Cells were maintained in RPMI-1640 supplemented with 10% FCS, 1% penicillin/streptomycin, and 1% stable glutamine (PAN Biotech, Aidenbach, Germany).

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC
    Article Snippet: .. Cells were incubated at 37 °C and 5% CO 2 in a humidified incubator and centrifuged at 300 × g for 5 min. Human adenocarcinoma (A549, H838, H1650, H1975) and squamous cell carcinoma (H520) cell lines were purchased from the American Type Culture Collection (ATCC) and JURKAT cells from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany). .. Cells were maintained in RPMI-1640 supplemented with 10% FCS, 1% penicillin/streptomycin, and 1% stable glutamine (PAN Biotech, Aidenbach, Germany).

    other:

    Article Title: Rational design of environmentally responsive antibodies with pH-sensing synthetic amino acids
    Article Snippet: Human adenocarcinoma (SK-BR-3) cell lines, human epidermoid carcinoma (A431) cell lines and human Burkitt’s lymphoma (Raji) cell lines were obtained from ATCC (VA, USA).

    Article Title: Rational design of environmentally responsive antibodies with pH-sensing synthetic amino acids.
    Article Snippet: Human adenocarcinoma (SK-BR-3) cell lines, human epidermoid carcinoma (A431) cell lines and human Burkitt’s lymphoma (Raji) cell lines were obtained from ATCC (VA, USA).

    Derivative Assay:

    Article Title: Influence of host cell line and microsporidian species in the in vitro infection efficiency of Encephalitozoon spp.
    Article Snippet: .. HT-29 cells (ATCC HTB-38) and HCT 116 cells (ATCC CCL-247) are derived from human adenocarcinoma and colorectal carcinoma, and exhibit an epithelial morphology. .. Both cell lines were cultured at 37 °C, under 5% CO 2 in McCoy’s 5A medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Dutscher, Bernolsheim, France), 100 U/mL penicillin, 0.1 mg/mL streptomycin, and 0.25 μg/mL amphotericin B (Cytiva, Marlborough, MA, USA).



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    Image Search Results


    The viability of HT‐29 (A) and HDFa (B) cells was assessed following treatment with 3a–3d at concentrations of 3.125, 6.25, 12.5, 25, 50, and 100 µM. Cells were incubated with the compounds for either 24 or 48 h. Data are presented as mean ± SD ( n = 3). Statistical significance was defined as * p < 0.05 compared with the control group.

    Journal: Chemistry & Biodiversity

    Article Title: Integrated Biological Evaluation of Benzimidazolium–Chalcone Derivatives: Antioxidant, Anti‐AChE, Anticancer, and Anti‐MRSA Activities With Docking Insights

    doi: 10.1002/cbdv.71828

    Figure Lengend Snippet: The viability of HT‐29 (A) and HDFa (B) cells was assessed following treatment with 3a–3d at concentrations of 3.125, 6.25, 12.5, 25, 50, and 100 µM. Cells were incubated with the compounds for either 24 or 48 h. Data are presented as mean ± SD ( n = 3). Statistical significance was defined as * p < 0.05 compared with the control group.

    Article Snippet: The experiments were conducted using the human colorectal adenocarcinoma cell line HT‐29 (ATCC HTB‐38; RRID: CVCL_0320) and normal adult human primary dermal fibroblasts (HDFa; ATCC PCS‐201‐012; RRID: CVCL_PCS‐201‐012).

    Techniques: Incubation, Control

    IC 50 values were determined using the MTT assay in  HT‐29  and HDFA cells following treatment with compounds 3a–3d for 24 and 48 h.

    Journal: Chemistry & Biodiversity

    Article Title: Integrated Biological Evaluation of Benzimidazolium–Chalcone Derivatives: Antioxidant, Anti‐AChE, Anticancer, and Anti‐MRSA Activities With Docking Insights

    doi: 10.1002/cbdv.71828

    Figure Lengend Snippet: IC 50 values were determined using the MTT assay in HT‐29 and HDFA cells following treatment with compounds 3a–3d for 24 and 48 h.

    Article Snippet: The experiments were conducted using the human colorectal adenocarcinoma cell line HT‐29 (ATCC HTB‐38; RRID: CVCL_0320) and normal adult human primary dermal fibroblasts (HDFa; ATCC PCS‐201‐012; RRID: CVCL_PCS‐201‐012).

    Techniques: MTT Assay

    Flow cytometric analysis of HT‐29 cells treated with culture medium (A), 3a (B), 3b (C), 3c (D), and 3d (E) showing quadrant graphs of live cells (Q3), early apoptotic (Q1), late apoptotic (Q2), and necrotic (Q4). (F) Graph illustrating the proportions of viable cells, necrosis, and apoptosis in HT29 cells after treatment with compounds. Data are presented as mean ± standard deviation (SD). Asterisks indicate statistically significant differences relative to the control group ( * p < 0.05).

    Journal: Chemistry & Biodiversity

    Article Title: Integrated Biological Evaluation of Benzimidazolium–Chalcone Derivatives: Antioxidant, Anti‐AChE, Anticancer, and Anti‐MRSA Activities With Docking Insights

    doi: 10.1002/cbdv.71828

    Figure Lengend Snippet: Flow cytometric analysis of HT‐29 cells treated with culture medium (A), 3a (B), 3b (C), 3c (D), and 3d (E) showing quadrant graphs of live cells (Q3), early apoptotic (Q1), late apoptotic (Q2), and necrotic (Q4). (F) Graph illustrating the proportions of viable cells, necrosis, and apoptosis in HT29 cells after treatment with compounds. Data are presented as mean ± standard deviation (SD). Asterisks indicate statistically significant differences relative to the control group ( * p < 0.05).

    Article Snippet: The experiments were conducted using the human colorectal adenocarcinoma cell line HT‐29 (ATCC HTB‐38; RRID: CVCL_0320) and normal adult human primary dermal fibroblasts (HDFa; ATCC PCS‐201‐012; RRID: CVCL_PCS‐201‐012).

    Techniques: Analysis, Standard Deviation, Control

    Sensitivity (IC 50 ) of Breast Cancer Cell Lines and Normal Human Fibroblasts to UA Nanoformulations, UA Carrier, and Free UA

    Journal: International Journal of Nanomedicine

    Article Title: PVP-Stabilized Ag@SiO 2 Nanocarriers for Usnic Acid: Physicochemical Characterization and Exploratory in vitro Anticancer, Antimicrobial, and Immunomodulatory Activity

    doi: 10.2147/IJN.S629377

    Figure Lengend Snippet: Sensitivity (IC 50 ) of Breast Cancer Cell Lines and Normal Human Fibroblasts to UA Nanoformulations, UA Carrier, and Free UA

    Article Snippet: Human breast adenocarcinoma cell lines MCF-7 and MDA-MB-231 were obtained from CLS Cell Lines Service GmbH (Eppelheim, Germany; cat. no. 86012803 for MCF-7 and cat. no. 92020424 for MDA-MB-231).

    Techniques: Sensitivity

    Intracellular ROS accumulation after 24 h treatment with the UA nanoformulation or free UA in MCF-7 ( A ) and MDA-MB-231 ( B ) breast cancer cell lines. Data are presented as mean ± SD from at least three independent experiments. Statistical significance is indicated. *p < 0.05, **p < 0.01, ***p < 0.001 .

    Journal: International Journal of Nanomedicine

    Article Title: PVP-Stabilized Ag@SiO 2 Nanocarriers for Usnic Acid: Physicochemical Characterization and Exploratory in vitro Anticancer, Antimicrobial, and Immunomodulatory Activity

    doi: 10.2147/IJN.S629377

    Figure Lengend Snippet: Intracellular ROS accumulation after 24 h treatment with the UA nanoformulation or free UA in MCF-7 ( A ) and MDA-MB-231 ( B ) breast cancer cell lines. Data are presented as mean ± SD from at least three independent experiments. Statistical significance is indicated. *p < 0.05, **p < 0.01, ***p < 0.001 .

    Article Snippet: Human breast adenocarcinoma cell lines MCF-7 and MDA-MB-231 were obtained from CLS Cell Lines Service GmbH (Eppelheim, Germany; cat. no. 86012803 for MCF-7 and cat. no. 92020424 for MDA-MB-231).

    Techniques: